The College of Science for Women at the University of Baghdad examined a master’s thesis entitled ” Synergistic Antileishmanial Activity of Ag: Se Nanoparticles Synthesized with Plantago lanceolata. A Novel Combination Therapy for Leishmania spp.”, presented by the postgraduate student Zahraa Basheer Jaber as a requirement for the degree of Master of Science in Biology/Zoology. The study was supervised by Prof. Dr. Naksheen Merza Mahmood
This study aimed to evaluate the synergistic antileishmanial activity of Ag: Se nanoparticles synthesized using Plantago Lanceolate and the individual effects of these nanoparticles and the extract. Additionally, the cytotoxicity of these treatments on normal rat embryo fibroblast REF cell lines was assessed.
The results revealed No significant differences were observed across treatments after 1 hour. The concentration of 100% of Ag: Se NPs at 250 g mL-1 had the highest inhibition effect (30.4%) on L. tropica. The highest effect was observed at a concentration of 100% for Ag: Se (125, 250) μg mL-1combined with extract 250 μg mL-1 after 48 hours of exposure, achieving growth inhibition rates of 59.6 % on L. donovani. No cytotoxicity was observed against a normal cell line using several dilute concentrations of Ag: Se NPs (25, 50, and 100%) and P. lanceolata concentrations (125 and 250) μg mL-1.
Future studies should explore the in vivo effects of Ag: Se nanoparticles, both individually and in combination with Plantago lanceolata extract, on Leishmania tropica and Leishmania donovani, using varying concentrations for a comprehensive understanding.
Final Grade: Excellent.
