College of Science for Women / University of Baghdad examined the thesis (evaluation of the effect of some antimicrobial agents on pathogenic staphylococci isolated from patients with fractures) of the student Zahraa Mohammed Fadhil.
The thesis aims to investigate the effect of antimicrobial agents on the susceptibility pattern of Staphylococcus species.
The thesis includes a total of 104 clinical samples that were isolated from patients with fractures, and 39 were identified as Staphylococcus species by the Vitek2 compact system. In general, (12) isolates belong to S. aureus, (8) isolates belong to S. epidermidis, (5) S. warneri, (4) isolates of S. hominis, and S. lentus, (3) isolates belong to S. haemolyticus, (1) isolate of which S. sciuri, S. lugdunensis, and S. pseudintermedius. Investigate the susceptibility of isolates of Staphylococcus species using microtiter dilution assay with nitrofurantoin, colistin, sulbactam, and colistin-sulbactam combination. Examined by quantitive PCR analysis (qPCR) the involvement of nitrofurantoin on the expression level of the msrA, mepA, qacAB, and qacC genes, which reveals a remarkable overexpression of qacC in S. haemolyticus.
The most important recommendations which the study has come up:
- Investigating the impact of combining Colistin with Sulbactam on pathogenic Gram-negative bacteria.
- Further exploring the mechanisms of nitrofurantoin resistance, including the prevalence of genes such as msrA, mepA, qacAB, and qacC among the isolated bacterial strains.
- Explore alternative antibiotics or antimicrobial agents that could be effective in combination with Sulbactam or Colistin, particularly for multi-drug resistant strains.
- Studying the potential cytotoxicity or side effects of combining Colistin and Sulbactam in clinical settings.
- Adopting the results of the current study in therapeutic applications against pathogenic bacteria.
The student obtained an excellent grade.
