1. To determine the serum levels of heavy metals, including Ni, Pb, Cd, and Cr, in asthma patients and healthy individuals.
2. To compare heavy metal levels among patients with moderate asthma, severe asthma, and the healthy control group.
3. To determine the relationship between heavy metal levels and asthma severity.

This study was conducted at the University of Baghdad / College of Science for Women / Department of Chemistry, in collaboration with Baghdad Teaching Hospital and Al-Zahraa Specialized Center for Allergy and Respiratory Diseases in Baghdad, Iraq, from September to November. The concentrations of heavy metals Pb, Cd, Cr, and Ni were measured using ICP-OES after conventional wet acid digestion of the samples, aiming to investigate the relationship between heavy metal exposure and asthma severity.

1. Conduct future studies with larger sample sizes and from different geographical regions to evaluate the relationship between heavy metal exposure and asthma severity.
2. Expand future studies to include other heavy metals, such as mercury, arsenic, and manganese, to provide a more comprehensive assessment of the effects of environmental exposure in asthma patients.
3. Investigate the relationship between serum heavy metal concentrations and lung function parameters, such as FEV1, FVC, and FEV1/FVC, to assess their association with asthma severity.
4. Compare ICP-OES with more advanced analytical techniques, such as ICP-MS, to improve sensitivity and detection limits for heavy metal analysis and assess measurement accuracy.