The thesis was conducted to to prepare and characterize nanostructured lipid carriers (NLCs) loaded with the bioactive components of plant extracts to enhance their antimicrobial properties and activities. Also evaluate their activities in– vitro and in-vivo studies against biofilm formation – MDR pathogens that isolated from oral cavity of apparently healthy and unhealthy patients from different hospitals in Baghdad.

The study includes conducting a research study on oral diseases. Oral cavity samples (100) of different ages were collected from apparently healthy and unhealthy patients suffering from dental caries or cavities, gingivitis, periodontal disease, and thrush from various hospitals in Baghdad during the period from November 2022 to June 2023.

The study concluded that all 32 isolates of S. aureus, P. aeruginosa, and C. albicans were identified as having high multidrug resistance patterns using the Vitec-2 system. The plant extracts eugenol and glycyrrhizic acid (GA) in the form of nanostructures were recorded. The best results were obtained by characterizing the EGAE-NLCs using a series of biochemical reactions and analyzing them using X-ray diffraction (XRD), scanning electron microscopy (SEM), particle size analysis, energy-dispersive X-ray (EDX), and atomic force microscopy (AFM). Characterization of the prepared oral EGAE-NLCs gel showed good physical and chemical stability throughout the study period. The results of the application of the oral EGAE-NLCs gel in histopathological studies showed high efficacy in treating mice infected with isolates of MRSA, P. aeruginosa, and C. albicans, suggesting its potential as an alternative treatment for multidrug-resistant microbial infections and biofilm-associated oral infections.

Excellent