Thesis Discussion
The College of Science for Women / University of Baghdad examined the thesis entitled (Evaluation of Immunological,Hematological and Hormonal markerﹸs inPlaque Psoriasis Iraqi Patients) of the student (Aaraf Harith Abdul Wahhab), in Partial Fulfilment of the Requirements for the Degree Doctor of Philosophy in Biology/Microbiology
By the student: Aseel Hikmat Abd-Alameer
The study’s objectives are to assess the levels of IL-10,IL-22,IL36 and CD3,CD4,CD8 in the blood of Patients of psoriasis and to investigate their potential association with the disease. It also looks at hematological factors to see if any changes are specific to the disease. Additionally, the study looks into the statistical link between psoriasis and subclinical hormones by checking thyroid function tests T3, T4, TSH, cortisol levels , also looked at to see how the correlation with psoriasis.
The study included collecting blood samples from 100 patients and 40 controls and conducting blood analysis to detect the numbers of white blood cells, hemoglobin, platelets, and red blood cells and comparing them with the control group. Also, conducting an immune examination for patients to detect interleukin 10, interleukin 22, and interleukin 36 by ELISA examination, and analyzing CD3, CD4, and CD8 by Flow Cytometry device and comparing them with the control group. Also, conducting a hormonal analysis for thyroid hormones and cortisol for patients and comparing them with the control group, and then finding the relationship between all immune indicators.
The present investigation revealed that the females were more frequently observed than Males among psoriasis patients and Control. Psoriasis patients exhibited significantly higher (BMI) compared to healthy control groups. The age group 31-45 year is the most frequent with psoriasis. Stress was more frequently shown in patients in comparison with control.There were significant differences in total WBCs Counts, Neutrophils %, Lymphocytes %, Eosinophil’s %, Monocytes % and Basophil % in patients group compared with healthy control group.Platelet count showed significant variation across the age groups. The highest mean PLT was observed in the 46-60 age group.Immunologically, interleukin-10 (IL-10) levels were markedly lower in patients in comparison with controls Conversely, interleukin-22 (IL-22) and interleukin-36 (IL-36) levels were significantly higher in patients.Regarding hormonal analysis, there were no significant differences in T3, T4, or TSH levels between patients and controls while , cortisol levels were significantly lower in patients. CD3, CD4, and CD8 percentages in blood have a significant difference between the patients and healthy control group .
The final grade: VeryGood
