The objectives of this study are summarized as follows:

1. To evaluate the potential use of 14-3-3 eta protein, CTX-II, and Anti-CCP as biomarkers in the serum of Iraqi women patients with rheumatoid arthritis.
2. To determine the correlation between 14-3-3 eta protein and CTX-II in serums of Iraqi patients with rheumatoid arthritis and other clinical and biochemical parameters.

Other parameters, such as CRP, RF, CBC, ESR, and lipid profile, were also measured in both the patient and healthy control groups.

This study, which was conducted at the University of Baghdad / College of Science for Women / Department of Chemistry and Al Yarmouk Teaching Hospital and Al-Kadhimiya Hospital in Baghdad, Iraq, between August to November 2024. Levels of 14-3-3 eta protein, CTX-II, anti-CCP antibodies, CRP and RF were measured using ELISA via the Human Reader HS device, while a complete blood count (CBC) was performed using a Sysmex XN-1000 analyzer, and the Westergren method was adopted for ESR analysis.

The most important recommendations which the study has come up with and the average obtained:

1. 1. 1. Examining the functions of CTX-II and 14-3-3 eta protein in RA patients receiving various forms of treatment, taking treatment duration into account, to gain a better understanding of how these proteins affect disease outcomes.
      2. Learning more about how these criteria are used to diagnose additional conditions systemic lupus erythematosus, and familial Mediterranean fever (FMF) illness, more research is required.
      3. The study recommends adding more male samples for better comparison validity and comprehensiveness, as well as larger patient and controls samples for more lucid results.
      4. Study the effect of duration of disease on patients with RA.