The College of Science for Women at the University of Baghdad discussed a master’s thesis entitled “Antibacterial and Anti-biofilm Efficacy of Biosynthesized Silver Nanoparticles against Clinical Isolates of Metallo-Beta-Lactamase and Extended-Spectrum Beta-Lactamase Producing Pseudomonas aeruginosa” Which was submitted by the student Marwa Mohammed Safih to obtain a master’s degree in life sciences/microbiology, under the supervision of Dr. Huda Suhail Abid.
The thesis focused on isolating and diagnosing clinical bacterial isolates from Iraqi patients from Baghdad hospitals and evaluated their antibiotic resistance. Also, Berberis vulgaris extracts were evaluated for their antibacterial properties against Metallo-Beta-Lactamase and Extended-Spectrum beta-lactamase-producing Pseudomonas aeruginosa obtained from clinical sources. The results showed that Berberis vulgaris extracts have antibacterial activity against the selected bacterial isolates. The total amount of chemical compounds in this plant was determined using (GC-MS) examination and through the examination, the presence of terpenes and flavonoids was detected.
Involved in the biosynthesis of silver nanoparticles by the green method using Berberis vulgaris extract. The characterization of silver nanoparticles (AgNPs) was performed through various modern techniques, including X-ray diffraction meter (XRD), scanning electron microscopy (SEM), atomic microscopy (AFM), and energy-dispersing X-ray spectrometry (EDX). The antibacterial activity of silver nanoparticles against bacterial isolates was studied, and the results indicated that (AgNPs) show antibacterial activity against P. aeruginosa. The inhibitory effect of B. vulgaris extract and AgNPs on the biofilm formation ability of bacterial isolates was also tested by the Microtiter plate assay using Sub-MIC concentration obtained from the antibacterial effect study of B.vulgaris extract and AgNPs, the results showed inhibitory efficacy against biofilm virulence factor.
The study recommended the clinical application of biosynthesized silver nanoparticles from the Berberis vulgaris extract as an alternative to conventional antibiotics and the possibility of manufacturing ointment from extracts of the B. Vulgaris or silver nanoparticles for the treatment of burns and superficial wounds.
Final Grade: Excellence.
