The College of Science for women in University of Baghdad discussed a master’s thesis entitled ” Bioactivity of Biosynthesized Selenium Nanoparticles Using Lactobacillus plantarum against Some Antibiotic-Resistant Pathogenic Clinical Bacterial Isolates “, which was submitted by the student Nuha Khaldoon Atta, Master’s degree in in Biology/Microbiology , Supervised by Assist. Prof. Dr. Huda Suhail Abid
Concentrated thesis isolation and identification of multidrug-resistant bacterial isolates from Iraqi patients from Baghdad city hospitals. Local dairy products were also used to isolate Lactobacillus plantarum, which were then evaluated for their antibacterial properties against multidrug-resistant Staphylococcus aureus and Pseudomonas aeruginosa obtained from clinical sources. The results showed that L. plantarum extract has antibacterial activity against the selected bacterial isolates. The total amount of phenolic compounds in the crude lactobacilli extract was determined using the standard Folin-Ciocalteu reagent. The total flavonoid content of the crude extract was investigated using the colorimetric aluminum chloride method, and the analysis revealed the presence of phenols and flavonoids
The biosynthesis of selenium nanoparticles was performed using the green method, specifically through Lactobacillus plantarum extract. The characterization of selenium nanoparticles (SeNPs) was performed by various modern techniques, including UV-Vis absorption spectroscopy, X-ray diffractometer (XRD), Scanning electron microscopy (SEM), Atomic force microscopy (AFM), Fourier transform infrared spectrometer (FTIR), Energy-dispersive X-ray spectroscopy (EDX), Atomic absorption spectrophotometer (AAS), and Zeta potential analyzer. The antibacterial activity of selenium nanoparticles against bacterial isolates was studied, and the results indicated that SeNPs exhibit antibacterial activity against S. aureus and P. aeruginosa, and the efficacy of selenium nanoparticles against Gram-positive bacteria was more than that against Gram-negative bacteria. The inhibitory effect of L. plantarum and SeNPs against biofilm formation by bacterial isolates was also tested by Microtiter plate assay using Sub-MIC concentration to study the antibacterial effect of Lactobacillus plantarum extract and SeNPs, and the results showed inhibitory activity against the virulence factor of biofilms.
The study recommended the possibility of using biosynthesized selenium nanoparticles from lactobacilli clinically as an alternative to traditional antibiotics, and the possibility of manufacturing an ointment from lactobacilli extracts or selenium nanoparticles to treat burns and superficial wounds.
Final Grade: Excellent.
