{"id":12274,"date":"2026-04-15T10:16:18","date_gmt":"2026-04-15T07:16:18","guid":{"rendered":"https:\/\/en.csw.uobaghdad.edu.iq\/?p=12274"},"modified":"2026-04-15T10:40:12","modified_gmt":"2026-04-15T07:40:12","slug":"masters-thesis-discussion-at-the-college-of-science-for-women-on-a-application-of-plant-tissue-culture-techniques-in-the-propagation-of-tulip-tulipa-gesneriana-l-2","status":"publish","type":"post","link":"https:\/\/en.csw.uobaghdad.edu.iq\/?p=12274","title":{"rendered":"(Association of Genetic Variation and Gene Expression of the PVC Operon with Biofilm Formation in Clinical Isolates of Pseudomonas aeruginosa)"},"content":{"rendered":"

[vc_row full_width=”stretch_row” rtl_reverse=”yes” css=”.vc_custom_1726859899671{margin-top: -150px !important;padding-top: 150px !important;padding-bottom: 60px !important;background-color: #f5f5f5 !important;}”][vc_column width=”1\/2″]

<\/div>

Master\u2019s<\/span> Discussion<\/span><\/span><\/span><\/p>\n

\u00a0The College of Science for Women \/ University of Baghdad examined the thesis entitled (Association of Genetic Variation and Gene Expression of the PVC<\/em> Operon with Biofilm Formation in Clinical Isolates of Pseudomonas aeruginosa<\/em><\/strong>) of the student (Zena Loay Abdul Jabbar<\/strong>), in Partial Fulfilment of the Requirements for the Degree of Master in Biology \/ Genetics.<\/p>\n<\/div><\/div><\/div>

<\/div><\/div>
<\/div>
<\/div>
<\/i>
Objectives of the Thesis<\/div><\/span>
[vc_column_text css=””]The study aims to determine the relationship between gene expression patterns of the pvcABCD<\/em> gene cluster<\/strong>\u00a0in clinical isolates of Pseudomonas aeruginosa<\/em>\u00a0from different infection sources, and the genetic variations associated with biofilm formation and antibiotic resistance.[\/vc_column_text]<\/div><\/div>
<\/i>
Content of the Thesis<\/div><\/span>
[vc_column_text css=””]This study includes the analysis of gene expression and genetic variation of the pvcABCD <\/em>operon in Pseudomonas aeruginosa<\/em> isolated from patients with burns, wounds, diabetic foot ulcers, in addition to urine samples. The study was conducted in Baghdad, where 137 clinical specimens were collected from different sources. The isolates were identified using bacteriological methods and confirmed by molecular techniques. Furthermore, genetic variation in the pvcA<\/em> and pvcC<\/em> genes was investigated in a number of isolates and compared with reference strains.[\/vc_column_text]<\/div><\/div>
<\/i>
recommendations<\/div><\/span>
[vc_column_text css=””]The most important recommendations which the study has come up with and the average obtained<\/strong>:<\/p>\n
    \n
  1. The study concluded that there is a clear variation in the gene expression levels of the pvcABCD<\/em> operon in Pseudomonas aeruginosa<\/em>, where a general decrease in the gene expression of the pvcA<\/em> gene was recorded, while the pvcB, pvcC<\/em>, and pvcD<\/em> genes showed a noticeable increase in gene expression. The results also showed a relationship between the high gene expression of this operon\u2019s genes and the increased ability of the isolates to form biofilms, especially in strong and moderate types. The results also showed differences in gene expression according to the source of the isolate, where urine isolates recorded the highest expression of the pvcA<\/em> gene, while burn and diabetic foot ulcer isolates showed an increase in the gene expression of the remaining operon genes compared to wound isolates, which showed a general decrease. The study of genetic variation also revealed the presence of missense and silent mutations in the pvcA<\/em> gene, which were associated with an increase in gene expression levels, indicating the role of compensatory regulatory mechanisms, while pvcC<\/em> showed high genetic stability. These results indicate an important role of the pvcABCD<\/em> operon in enhancing bacterial adaptation and virulence.<\/li>\n<\/ol>\n

    [\/vc_column_text]<\/div><\/div>

    <\/i>
    The final grade<\/div><\/span>
    [vc_column_text css=””]The final grade: Excellent <\/strong>[\/vc_column_text]<\/div><\/div>
    <\/i>
    Picture of the Committee members<\/div><\/span>
    [vc_column_text css=””]<\/p>\n
    \n
    \n
    \"\"<\/div>\n<\/div>\n<\/div>\n

    [\/vc_column_text]<\/div><\/div><\/div><\/div>[vc_raw_js]JTNDc2NyaXB0JTNFJTIwdmFyJTIwdHJhbnNfcGFnZSUzRCUyMjUyNTE2JTIyJTIwJTNDJTJGc2NyaXB0JTNF[\/vc_raw_js][\/vc_column][vc_column width=”1\/2″]

    <\/div>[vc_single_image image=”12280″ img_size=”700×700″ alignment=”right” css=””][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"

    [vc_row full_width=”stretch_row” rtl_reverse=”yes” css=”.vc_custom_1726859899671{margin-top: -150px !important;padding-top: 150px !important;padding-bottom: 60px !important;background-color: #f5f5f5 !important;}”][vc_column width=”1\/2″][vc_raw_js]JTNDc2NyaXB0JTNFJTIwdmFyJTIwdHJhbnNfcGFnZSUzRCUyMjUyNTE2JTIyJTIwJTNDJTJGc2NyaXB0JTNF[\/vc_raw_js][\/vc_column][vc_column width=”1\/2″][vc_single_image image=”12280″ img_size=”700×700″ alignment=”right” css=””][\/vc_column][\/vc_row] … <\/p>\n","protected":false},"author":29,"featured_media":12280,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_lmt_disableupdate":"no","_lmt_disable":"","footnotes":""},"categories":[15,12],"tags":[],"class_list":["post-12274","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-masters","category-postgraduate-discussions"],"yoast_head":"\n(Association of Genetic Variation and Gene Expression of the PVC Operon with Biofilm Formation in Clinical Isolates of Pseudomonas aeruginosa) - College of Science for Women<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/en.csw.uobaghdad.edu.iq\/?p=12274\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"(Association of Genetic Variation and Gene Expression of the PVC Operon with Biofilm Formation in Clinical Isolates of Pseudomonas aeruginosa) - College of Science for Women\" \/>\n<meta property=\"og:description\" content=\"[vc_row full_width=”stretch_row” rtl_reverse=”yes” css=”.vc_custom_1726859899671{margin-top: -150px !important;padding-top: 150px !important;padding-bottom: 60px !important;background-color: #f5f5f5 !important;}”][vc_column width=”1\/2″][vc_raw_js]JTNDc2NyaXB0JTNFJTIwdmFyJTIwdHJhbnNfcGFnZSUzRCUyMjUyNTE2JTIyJTIwJTNDJTJGc2NyaXB0JTNF[\/vc_raw_js][\/vc_column][vc_column width=”1\/2″][vc_single_image image=”12280″ img_size=”700×700″ alignment=”right” css=””][\/vc_column][\/vc_row] ...\" \/>\n<meta property=\"og:url\" content=\"https:\/\/en.csw.uobaghdad.edu.iq\/?p=12274\" \/>\n<meta property=\"og:site_name\" content=\"College of Science for Women\" \/>\n<meta property=\"article:published_time\" content=\"2026-04-15T07:16:18+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2026-04-15T07:40:12+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/en.csw.uobaghdad.edu.iq\/wp-content\/uploads\/sites\/97\/2026\/04\/photo_2026-03-29_08-10-49.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1920\" \/>\n\t<meta property=\"og:image:height\" content=\"2560\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"csw\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"csw\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"2 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/en.csw.uobaghdad.edu.iq\/?p=12274\",\"url\":\"https:\/\/en.csw.uobaghdad.edu.iq\/?p=12274\",\"name\":\"(Association of Genetic Variation and Gene Expression of the PVC Operon with Biofilm Formation in Clinical Isolates of Pseudomonas aeruginosa) - College of Science for Women\",\"isPartOf\":{\"@id\":\"https:\/\/en.csw.uobaghdad.edu.iq\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\/\/en.csw.uobaghdad.edu.iq\/?p=12274#primaryimage\"},\"image\":{\"@id\":\"https:\/\/en.csw.uobaghdad.edu.iq\/?p=12274#primaryimage\"},\"thumbnailUrl\":\"https:\/\/en.csw.uobaghdad.edu.iq\/wp-content\/uploads\/sites\/97\/2026\/04\/photo_2026-03-29_08-10-49.jpg\",\"datePublished\":\"2026-04-15T07:16:18+00:00\",\"dateModified\":\"2026-04-15T07:40:12+00:00\",\"author\":{\"@id\":\"https:\/\/en.csw.uobaghdad.edu.iq\/#\/schema\/person\/e2d8d23f32d2810ed05a93fd995a99de\"},\"breadcrumb\":{\"@id\":\"https:\/\/en.csw.uobaghdad.edu.iq\/?p=12274#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/en.csw.uobaghdad.edu.iq\/?p=12274\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/en.csw.uobaghdad.edu.iq\/?p=12274#primaryimage\",\"url\":\"https:\/\/en.csw.uobaghdad.edu.iq\/wp-content\/uploads\/sites\/97\/2026\/04\/photo_2026-03-29_08-10-49.jpg\",\"contentUrl\":\"https:\/\/en.csw.uobaghdad.edu.iq\/wp-content\/uploads\/sites\/97\/2026\/04\/photo_2026-03-29_08-10-49.jpg\",\"width\":1920,\"height\":2560},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/en.csw.uobaghdad.edu.iq\/?p=12274#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\/\/en.csw.uobaghdad.edu.iq\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"(Association of Genetic Variation and Gene Expression of the PVC Operon with Biofilm Formation in Clinical Isolates of Pseudomonas aeruginosa)\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/en.csw.uobaghdad.edu.iq\/#website\",\"url\":\"https:\/\/en.csw.uobaghdad.edu.iq\/\",\"name\":\"College of Science for Women\",\"description\":\"University of baghdad\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/en.csw.uobaghdad.edu.iq\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"},{\"@type\":\"Person\",\"@id\":\"https:\/\/en.csw.uobaghdad.edu.iq\/#\/schema\/person\/e2d8d23f32d2810ed05a93fd995a99de\",\"name\":\"csw\",\"image\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/en.csw.uobaghdad.edu.iq\/#\/schema\/person\/image\/\",\"url\":\"https:\/\/secure.gravatar.com\/avatar\/975096ae94d110f34c0a04073463cc8b4e1348753830dceb23577827e607beec?s=96&d=mm&r=g\",\"contentUrl\":\"https:\/\/secure.gravatar.com\/avatar\/975096ae94d110f34c0a04073463cc8b4e1348753830dceb23577827e607beec?s=96&d=mm&r=g\",\"caption\":\"csw\"},\"url\":\"https:\/\/en.csw.uobaghdad.edu.iq\/?author=29\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"(Association of Genetic Variation and Gene Expression of the PVC Operon with Biofilm Formation in Clinical Isolates of Pseudomonas aeruginosa) - College of Science for Women","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/en.csw.uobaghdad.edu.iq\/?p=12274","og_locale":"en_US","og_type":"article","og_title":"(Association of Genetic Variation and Gene Expression of the PVC Operon with Biofilm Formation in Clinical Isolates of Pseudomonas aeruginosa) - College of Science for Women","og_description":"[vc_row full_width=”stretch_row” rtl_reverse=”yes” css=”.vc_custom_1726859899671{margin-top: -150px !important;padding-top: 150px !important;padding-bottom: 60px !important;background-color: #f5f5f5 !important;}”][vc_column width=”1\/2″][vc_raw_js]JTNDc2NyaXB0JTNFJTIwdmFyJTIwdHJhbnNfcGFnZSUzRCUyMjUyNTE2JTIyJTIwJTNDJTJGc2NyaXB0JTNF[\/vc_raw_js][\/vc_column][vc_column width=”1\/2″][vc_single_image image=”12280″ img_size=”700×700″ alignment=”right” css=””][\/vc_column][\/vc_row] ...","og_url":"https:\/\/en.csw.uobaghdad.edu.iq\/?p=12274","og_site_name":"College of Science for Women","article_published_time":"2026-04-15T07:16:18+00:00","article_modified_time":"2026-04-15T07:40:12+00:00","og_image":[{"width":1920,"height":2560,"url":"https:\/\/en.csw.uobaghdad.edu.iq\/wp-content\/uploads\/sites\/97\/2026\/04\/photo_2026-03-29_08-10-49.jpg","type":"image\/jpeg"}],"author":"csw","twitter_card":"summary_large_image","twitter_misc":{"Written by":"csw","Est. reading time":"2 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/en.csw.uobaghdad.edu.iq\/?p=12274","url":"https:\/\/en.csw.uobaghdad.edu.iq\/?p=12274","name":"(Association of Genetic Variation and Gene Expression of the PVC Operon with Biofilm Formation in Clinical Isolates of Pseudomonas aeruginosa) - College of Science for Women","isPartOf":{"@id":"https:\/\/en.csw.uobaghdad.edu.iq\/#website"},"primaryImageOfPage":{"@id":"https:\/\/en.csw.uobaghdad.edu.iq\/?p=12274#primaryimage"},"image":{"@id":"https:\/\/en.csw.uobaghdad.edu.iq\/?p=12274#primaryimage"},"thumbnailUrl":"https:\/\/en.csw.uobaghdad.edu.iq\/wp-content\/uploads\/sites\/97\/2026\/04\/photo_2026-03-29_08-10-49.jpg","datePublished":"2026-04-15T07:16:18+00:00","dateModified":"2026-04-15T07:40:12+00:00","author":{"@id":"https:\/\/en.csw.uobaghdad.edu.iq\/#\/schema\/person\/e2d8d23f32d2810ed05a93fd995a99de"},"breadcrumb":{"@id":"https:\/\/en.csw.uobaghdad.edu.iq\/?p=12274#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/en.csw.uobaghdad.edu.iq\/?p=12274"]}]},{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/en.csw.uobaghdad.edu.iq\/?p=12274#primaryimage","url":"https:\/\/en.csw.uobaghdad.edu.iq\/wp-content\/uploads\/sites\/97\/2026\/04\/photo_2026-03-29_08-10-49.jpg","contentUrl":"https:\/\/en.csw.uobaghdad.edu.iq\/wp-content\/uploads\/sites\/97\/2026\/04\/photo_2026-03-29_08-10-49.jpg","width":1920,"height":2560},{"@type":"BreadcrumbList","@id":"https:\/\/en.csw.uobaghdad.edu.iq\/?p=12274#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/en.csw.uobaghdad.edu.iq\/"},{"@type":"ListItem","position":2,"name":"(Association of Genetic Variation and Gene Expression of the PVC Operon with Biofilm Formation in Clinical Isolates of Pseudomonas aeruginosa)"}]},{"@type":"WebSite","@id":"https:\/\/en.csw.uobaghdad.edu.iq\/#website","url":"https:\/\/en.csw.uobaghdad.edu.iq\/","name":"College of Science for Women","description":"University of baghdad","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/en.csw.uobaghdad.edu.iq\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"},{"@type":"Person","@id":"https:\/\/en.csw.uobaghdad.edu.iq\/#\/schema\/person\/e2d8d23f32d2810ed05a93fd995a99de","name":"csw","image":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/en.csw.uobaghdad.edu.iq\/#\/schema\/person\/image\/","url":"https:\/\/secure.gravatar.com\/avatar\/975096ae94d110f34c0a04073463cc8b4e1348753830dceb23577827e607beec?s=96&d=mm&r=g","contentUrl":"https:\/\/secure.gravatar.com\/avatar\/975096ae94d110f34c0a04073463cc8b4e1348753830dceb23577827e607beec?s=96&d=mm&r=g","caption":"csw"},"url":"https:\/\/en.csw.uobaghdad.edu.iq\/?author=29"}]}},"modified_by":"csw","_links":{"self":[{"href":"https:\/\/en.csw.uobaghdad.edu.iq\/index.php?rest_route=\/wp\/v2\/posts\/12274","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/en.csw.uobaghdad.edu.iq\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/en.csw.uobaghdad.edu.iq\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/en.csw.uobaghdad.edu.iq\/index.php?rest_route=\/wp\/v2\/users\/29"}],"replies":[{"embeddable":true,"href":"https:\/\/en.csw.uobaghdad.edu.iq\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=12274"}],"version-history":[{"count":4,"href":"https:\/\/en.csw.uobaghdad.edu.iq\/index.php?rest_route=\/wp\/v2\/posts\/12274\/revisions"}],"predecessor-version":[{"id":12282,"href":"https:\/\/en.csw.uobaghdad.edu.iq\/index.php?rest_route=\/wp\/v2\/posts\/12274\/revisions\/12282"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/en.csw.uobaghdad.edu.iq\/index.php?rest_route=\/wp\/v2\/media\/12280"}],"wp:attachment":[{"href":"https:\/\/en.csw.uobaghdad.edu.iq\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=12274"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/en.csw.uobaghdad.edu.iq\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=12274"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/en.csw.uobaghdad.edu.iq\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=12274"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}